BPC-157 Reconstitution Guide: Proper Preparation for Research Use

Introduction

BPC-157 (Body Protection Compound-157) is a synthetic pentadecapeptide derived from a protective protein found in gastric juice. It is widely used in in-vitro research for its potential tissue-protective and regenerative properties. Before BPC-157 can be utilized in research protocols, it must be properly reconstituted from its lyophilized (freeze-dried) form.

This guide outlines the standard procedure for reconstituting BPC-157 peptide powder for laboratory use.

Required Materials

• Lyophilized BPC-157 peptide vial
• Bacteriostatic water (BAC water) or sterile water
• Alcohol swabs (70% isopropyl alcohol)
• Sterile syringes (1 mL insulin syringes recommended)
• Sterile work surface or laminar flow hood

Step-by-Step Reconstitution

Step 1: Prepare Your Workspace

Ensure your workspace is clean and sterile. If available, use a laminar flow hood. Wipe down surfaces with 70% isopropyl alcohol. Allow the peptide vial and solvent to reach room temperature before handling.

Step 2: Sanitize the Vial Tops

Swab the rubber stopper of both the BPC-157 vial and the bacteriostatic water vial with an alcohol wipe. Allow them to air dry for approximately 30 seconds.

Step 3: Draw the Solvent

Using a sterile syringe, draw the desired amount of bacteriostatic water. A common reconstitution volume for a 5 mg vial is 2 mL of BAC water, which yields a concentration of 2.5 mg/mL (2500 mcg/mL).

Step 4: Add Solvent to the Peptide

Insert the needle through the rubber stopper of the BPC-157 vial. Inject the water slowly along the inside wall of the vial. Do not spray directly onto the lyophilized powder, as this may damage the peptide structure.

Step 5: Allow Dissolution

Let the vial sit undisturbed for several minutes. The powder should dissolve on its own. If needed, gently roll the vial between your fingers. Never shake the vial, as vigorous agitation can denature the peptide.

Step 6: Verify the Solution

Once fully dissolved, the solution should be clear and free of visible particles. If the solution appears cloudy or contains undissolved material, allow additional time for dissolution.

Concentration Reference Table

| Vial Size | BAC Water | Concentration | |-----------|-----------|---------------| | 5 mg | 1 mL | 5000 mcg/mL | | 5 mg | 2 mL | 2500 mcg/mL | | 10 mg | 2 mL | 5000 mcg/mL | | 10 mg | 5 mL | 2000 mcg/mL |

Storage Recommendations

• Reconstituted BPC-157 should be stored at 2-8 degrees Celsius (standard refrigerator temperature)
• When reconstituted with bacteriostatic water, the solution remains stable for up to 28 days
• When reconstituted with sterile water (no preservative), use within 48 hours
• Lyophilized (unreconstituted) BPC-157 can be stored at -20 degrees Celsius for long-term stability
• Avoid repeated freeze-thaw cycles, as this degrades peptide integrity

Important Notes

• Always use aseptic technique when handling peptides
• Bacteriostatic water is preferred over sterile water for reconstitution due to the benzyl alcohol preservative, which inhibits microbial growth
• Label your vials with the reconstitution date, concentration, and peptide name
• All BPC-157 products are intended strictly for in-vitro research and laboratory use

Quality Assurance

When purchasing BPC-157 for research, always verify that the supplier provides a Certificate of Analysis (COA) from an independent testing laboratory. At PeptidesDirect, every batch is verified by Janoshik Analytical for purity and identity confirmation.