What is the BPC-157/TB-500 Mix?

The BPC-157/TB-500 Mix is a pre-blended combination of two well-researched peptides - BPC-157 and TB-500 (Thymosin Beta-4). This combination is studied for potential synergistic effects in tissue repair research.

How should I store the BPC-157/TB-500 Mix?

Store the lyophilized blend at -20°C for long-term storage or at 2-8°C for short-term use. Once reconstituted, refrigerate at 2-8°C and use within 30 days. Avoid repeated freeze-thaw cycles.

What purity is the BPC-157/TB-500 Mix?

Each component in our BPC-157/TB-500 Mix is independently tested by Janoshik Analytical with verified purity of 98% or higher. Certificates of analysis are available on the product page.

How do I reconstitute the BPC-157/TB-500 Mix?

Add bacteriostatic water slowly along the inside wall of the vial. Gently swirl until fully dissolved - do not shake. Use 1-2 mL of bacteriostatic water. The solution should be clear before use.

BPC-157/TB-500 Mix Peptide Blend

Name: WOLVERINE (BPC-157 + TB-500)
Brand: PeptidesDirect
SKU: PD-BPC157TB500MIX-10MG
Price: 59.99 EUR
Availability: InStock

Two peptides, one vial

A pre-mixed blend of BPC-157 and TB-500, the two most studied regenerative peptides in tissue-repair literature. Often called the "Wolverine Stack" by researchers.

BPC-157: vessels and gut

A 15-amino-acid pentadecapeptide first isolated from gastric juice. In animal models it promotes new blood-vessel formation, supports endothelial nitric-oxide signaling, and accelerates wound closure.

TB-500: actin and migration

A synthetic fragment of thymosin beta-4. Binds G-actin and helps regulate the cytoskeleton, the cellular machinery that lets cells crawl into a wound and rebuild tissue.

Tendon and soft-tissue research

Both peptides appear in the published literature on tendon, ligament, muscle, and skin repair models. The blend is widely used in combination-peptide research on musculoskeletal injury.

Calms inflammation

In animal and cell-culture studies, both peptides reduce pro-inflammatory cytokines such as TNF-alpha and IL-6, dampening the inflammatory phase of the repair cascade.

Complementary mechanisms

BPC-157 acts mainly on growth-factor and angiogenic pathways (VEGFR2, eNOS). TB-500 acts on the actin cytoskeleton and cell migration. The two pathways are studied as complementary in tissue-repair research.

Research areas

Tissue regenerationWound healingTendon & ligamentAngiogenesisCell migrationAnti-inflammatory

What is BPC-157 + TB-500 Mix

BPC-157 + TB-500 Mix is a single lyophilized vial containing two synthetic research peptides at fixed ratio. BPC-157 is a 15-amino-acid pentadecapeptide derived from a protective protein found in gastric juice. TB-500 is a 7-amino-acid synthetic fragment of thymosin beta-4, a naturally occurring actin-binding peptide present in nearly every cell of the body.

The combination is often called the "Wolverine Stack" in research circles, named after the comic-book character known for rapid healing. It is the most popular combo in tissue-regeneration research, because the two peptides target different but complementary steps of the repair cascade.

We supply the blend in lyophilized (freeze-dried) form: the same molecules used in the published studies, pre-mixed at a research-standard ratio.

How it works

Tissue repair is not one event. It involves stopping inflammation, building new blood vessels, recruiting cells to the injury site, rebuilding extracellular matrix, and remodeling the new tissue. BPC-157 and TB-500 each cover a different part of that cascade.

BPC-157 acts mostly on the vascular and growth-factor side. It upregulates VEGFR2 and activates the Akt-eNOS pathway, supporting the formation of new capillaries and stabilizing blood-vessel function. It also raises growth-factor signals such as EGR-1 and FGF, and protects endothelial cells under stress in animal models.

TB-500 acts mostly on the cytoskeleton. It binds G-actin, the building block of the cellular skeleton, and regulates how much free actin is available when a cell needs to change shape, migrate, or divide. This matters for fibroblasts crawling into a wound, for endothelial cells forming new vessels, and for stem cells homing to damaged tissue.

Used together in research models, the two peptides cover both the structural side of repair (vessels, growth factors, BPC-157) and the cellular-mobility side (actin, migration, TB-500). Both also lower pro-inflammatory cytokines, which means the early inflammatory phase is dampened on two pathways at once.

Often studied alongside

BPC-157 and TB-500 are also studied individually, and many research protocols compare the single peptides against the blend to separate which effects come from which compound. Both are available in research-grade form below.

BPC-157 alone, the gastric pentadecapeptide

BPC-157regeneration

Gastric pentadecapeptide (15 amino acids) known for exceptional tissue repair properties. Promotes wound healing, angiogenesis, and cytoprotection across tendons, muscles, gut, and nerves. Over 30 years of preclinical research.

TB-500 alone, the thymosin beta-4 fragment

TB-500regeneration

Active fragment of Thymosin Beta-4, a naturally occurring repair protein. Promotes cell migration and new blood vessel formation for systemic tissue healing. Especially researched for muscle, tendon, and cardiac repair.

For research that adds a skin and anti-inflammatory layer on top of the regeneration stack, the KLOW blend pairs the same two peptides with GHK-Cu and KPV. KLOW is studied in protocols that target both deep-tissue repair and skin or mucosal surfaces.

Four-peptide blend: BPC-157 + TB-500 + GHK-Cu + KPV

KLOWregeneration

4-in-1 anti-aging peptide blend: GHK-Cu 50mg + BPC-157 10mg + TB-500 10mg + KPV 10mg. Targets collagen synthesis, tissue regeneration, skin repair, and anti-inflammatory pathways.

For reconstitution, the standard solvent in published protocols is bacteriostatic or sterile water.

Standard solvent for reconstitution

Bacteriostatic Wateraccessories

USP-grade sterile water with 0.9% benzyl alcohol - the standard solvent for reconstituting lyophilized peptides. Essential accessory for any peptide research. Each vial is sealed and ready to use.

Documentation

Material specification

Purity

≥98% (HPLC verified, each peptide)

Test method

HPLC + mass spectrometry

Form

Lyophilized powder, pre-mixed blend

Storage (sealed)

2 to 8 °C, light-protected

Storage (reconstituted)

2 to 8 °C, use within 4 weeks

CoA

Batch-specific, third-party (Janoshik)

Lab Report (COA)

Batch NumberBlue

Purity99.90%

Testing LabJanoshik

Test Date30 Oct 2025

Download COA

Selected research

Research use only

This material is sold strictly for in-vitro research and laboratory use. Not intended for human or animal consumption, medical, cosmetic, or household applications. Suitable only for professional laboratory environments.